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Fact: Cigarettes are used by a small fraction of teens, but tobacco addiction is a serious issue and help is available.

The forward primer for ITS gene PCR amplification is ITS1 (sequence: CCGTAGGTGAACCTGCGG), and the reverse primer is ITS4 (sequence: TCCTCCGCTTATTGATATGC), with an amplification length of approximately 500 bp

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The hazardous properties of chemicals cannot be sufficiently determined using only non-animal methods therefore animal testing is still required to determine certain human health and environmental data

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